The scientists at the German Center for Neurodegenerative Diseases (DZNE) and Charite – Universitatsmedizin Berlin isolated almost 600 different antibodies from the blood of individuals who had overcome COVID-19, the disease triggered by SARS-CoV-2.
The research, published in the journal Cell, also shows that some SARS-CoV-2 antibodies bind to tissue samples from various organs, which could potentially trigger undesired side effects.
By means of laboratory tests, they were able to narrow this number down to a few antibodies that were particularly effective at binding to the virus. The researchers then produced these antibodies artificially using cell cultures. The so-called neutralising antibodies bind to the virus, as crystallographic analysis reveals, and thus prevent the pathogen from entering cells and reproducing, they said.
In addition, virus recognition by antibodies helps immune cells to eliminate the pathogen. Studies in hamsters — which, like humans, are susceptible to infection by SARS-CoV-2 — confirmed the high efficacy of the selected antibodies.
“If the antibodies were given after infection, the hamsters developed mild disease symptoms at most. If the antibodies were applied preventively — before infection — the animals did not get sick,” said Jakob Kreye, coordinator of the research project.
The researchers noted that treating infectious diseases with antibodies has a long history. For COVID-19, this approach is also being investigated through the administration of plasma-derived from the blood of recovered patients. With the plasma, antibodies of donors are transferred, they said.
“Ideally, the most effective antibody is produced in a controlled manner on an industrial scale and in constant quality. This is the goal we are pursuing,” said Momsen Reincke, first author of the research.
“Three of our antibodies are particularly promising for clinical development,. Using these antibodies, we have started to develop a passive vaccination against SARS-CoV-2,” explained Harald Pruss, a research group leader at the DZNE and also a senior physician at Charite – Universitatsmedizin Berlin.
In addition to the treatment of patients, preventive protection of healthy individuals who have had contact with infected persons is also a potential application. How long the protection lasts will have to be investigated in clinical studies, the researchers said.
“This is because, unlike in active vaccination, passive vaccination involves the administration of ready-made antibodies, which are degraded after some time,” Pruss said. In general, the protection provided by a passive vaccination is less persistent than that provided by an active vaccination, the researchers said.
However, the effect of a passive vaccination is almost immediate, whereas with an active vaccination it has to build up first, they said.
“It would be best if both options were available so that a flexible response could be made depending on the situation,” Pruss added.
(Edits by EP News Bureau)