Chung-Ang University researchers develop novel DNA biosensor for early diagnosis of cervical cancer

Researchers from Chung-Ang University developed an electrochemical DNA biosensor with enhanced sensitivity for effective HPV detection with high specificity. They achieved this feat using a graphitic nano-onion/molybdenum disulfide nanosheet composite with improved conductive electron transfer compared to the nanosheet alone. Their breakthrough can open doors to the development of electrochemical biosensors for early diagnosis of various ailments.

Molybdenum disulfide (MoS₂) has recently garnered attention among materials science researchers owing to its ability to form two-dimensional nanosheets like graphene. The nanosheets are created by the stacking of S–Mo–S layers interacting via Van der Waals interactions. Additionally, the unique structural, optical, thermal, and electrochemical properties of MoS₂ have opened up multiple research avenues across several fields, including the development of biomolecule sensing and chemical detection platforms, optoelectronics, supercapacitors, and batteries.

Traditionally, carbon nanostructures have been employed as an immobilisation platform for DNA. In order to substitute carbon with MoS₂ as an effective electrochemical DNA sensor, the electrical conductivity of MoS₂ needs to be improved considerably. Against this backdrop, Associate Professor Eunah Kang and Youngjun Kim from the School of Chemical Engineering and Material Science at Chung-Ang University, Korea have recently come up with an elegant solution. The duo has developed an electrochemical DNA biosensor using a graphitic nano-onion/molybdenum disulfide (MoS₂) nanosheet composite, which effectively detects human papillomavirus (HPV)-16 and HPV-18, and can serve as an early diagnosis of cervical cancer.

“Nano-onions possess graphitic sp₂ structures and are derived from crystalline sp₃ nanodiamonds via thermal annealing or laser irradiation,” explains Dr Kang. Their breakthrough was published in the Journal of Nanobiotechnology on 10 June 2023.

The researcher duo prepared the novel electrode surface for probing DNA chemisorption by enabling chemical conjugation between two functional groups: acyl bonds on the surfaces of functionalised nano-onions and amine groups present on the modified MoS₂ nanosheets. Cyclic voltammetry experiments revealed that a 1:1 composite electrode had an improved rectangular shape compared to that of an MoS₂ nanosheet electrode. “This indicated the amorphous nature of the nano-onions with curved carbon layers that facilitated an enhancement in electronic conductivity compared to MoS₂ nanosheet alone,” highlights Dr Kang.

Additionally, the duo measured the sensitivity of their novel electrochemical DNA biosensor device towards HPV-16 and HPV-18 by employing differential pulse voltammetry (DPV) technique in the presence of methylene blue (MB) as a redox indicator. Dr Kang elaborates, “The DPV current peak was lowered after probe DNA chemisorption and target DNA hybridisation. Since the hybridised DNA was double-stranded, it induced less effective MB electrostatic intercalation, resulting in a lower oxidation peak.”

The duo found that, compared to the MoS₂ nanosheet electrode, the nano-onion/MoS₂ nanosheet composite electrode attained higher current peaks, indicating a greater change in the differential peak. This was attributed to an enhanced conductive electron transfer owing to the nano-onion.

Notably, the target DNAs produced from HPV-16 and HPV-18 Siha and Hela cancer cell lines were detected by the proposed sensor effectively and with high specificity. Consequently, MoS₂ nanosheets with improved electrical conductivity facilitated by complexation with nano-onions provides a suitable platform for developing effective and efficient electrochemical biosensors for the early diagnosis of a wide variety of ailments, including cervical cancer.

Furthermore, combining nano-onions or nanodiamonds with different organic biomaterials can facilitate chemical functionality, electron transfer conductivity, light absorption, and more. These, in turn, can lead to innovative disease sensing, targeted drug delivery systems, and biomedical imaging and diagnostics.