During the COVID-19 pandemic (caused by the SARS[1]CoV-2 virus), wastewater has gained visibility as an alternative source of information for outbreak monitoring. SARS-CoV-2 RNA is shed in feces and other bodily fluids, and can therefore be recovered from wastewater. The detection of SARS-CoV-2 RNA in wastewater can be used as an early indicator of virus introduction to a population. Furthermore, SARS-CoV-2 RNA concentrations in wastewater mirror disease dynamics and can serve as a tool to monitor the progress of the pandemic. Because SARS-CoV-2 RNA concentrations in wastewater are typically low, wastewater samples must be concentrated prior to RNA extraction and quantification. Here we systematically tested the utility of ultracentrifugation as a method for virus concentration. We tested different initial wastewater volumes (min. 13 mL to max. 36 mL), different tube shapes (round bottom vs. conical), and different rotor types (fixed angle vs. swinging buckets), to identify the optimal concentration conditions. We then compared ultracentrifugation with other commonly used virus concentration methods (ultrafiltration, polyethylene glycol (PEG) precipitation, and organic flocculation).
• Product Interest
Centrifugation, Benchtop/General Purpose Centrifugation, High Performance Centrifugation, Ultracentrifugation
• Product Groups
Centrifugation, Genomics, High Performance, Table Top, Ultra Prep
• Methods
Centrifugation, Enrichments, Filtration – Chromatography, RNAseq, RT-PCR for RNA Biomolecules – Amplification, Separation
• Disease Research
COVID-19 Virus
• Disease Management
Monitoring
• Industry Segment
Aquaculture, Government, Public-Private Partnerships
• Sample Type
RNA – Nucleic Acids, Water
• Technology
Centrifugation, Ultra Harmonic Technology, Ultracentrifugation
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